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American Society of Microbiology ABMM Sample Questions:

1. A patient with HIV infection and a CD4+ T-cell count of 30 cells/µL develops progressive multifocal leukoencephalopathy (PML). The causative agent of PML is:

A) Cytomegalovirus (CMV)
B) Human herpesvirus 6 (HHV-6)
C) JC virus (JCV)
D) Epstein-Barr virus (EBV)

2. A microbiology laboratory is investigating a possible outbreak of Salmonella infection. Serological testing of patient isolates reveals they all possess the same O antigen but different H antigens. This suggests:

A) Multiple sources of infection with unrelated Salmonella strains.
B) Contamination in the laboratory affecting multiple samples.
C) A single point source of infection with a genetically stable strain.
D) A common ancestral strain that has undergone flagellar antigen variation.

3. A clinical microbiology laboratory is monitoring the emergence of antimicrobial resistance. The detection of a bacterial isolate with reduced susceptibility to multiple classes of antibiotics, often due to horizontal gene transfer of resistance determinants, is characteristic of:

A) A naturally competent organism.
B) A multidrug-resistant (MDR) organism.
C) An intrinsically resistant organism.
D) A pan-susceptible organism.

4. A patient with a history of neutropenia following chemotherapy develops a severe pneumonia.
Bronchoalveolar lavage fluid reveals the presence of hyaline, septate hyphae with frequent dichotomous branching at acute angles. Culture yields a filamentous fungus that produces conidiophores with a vesicle bearing phialides that release chains of conidia. The MOST likely genus is:

A) Fusarium
B) Rhizopus
C) Mucor
D) Aspergillus

5. A patient presents with a chronic, non-healing skin ulcer in a tropical region. Microscopic examination of a biopsy reveals amastigotes within macrophages. Culture of the biopsy on Novy-MacNeal-Nicolle (NNN) medium yields flagellated promastigotes. The MOST likely diagnosis is cutaneous leishmaniasis caused by:

A) Trypanosoma cruzi
B) Leishmania tropica or Leishmania major
C) Leishmania donovani
D) Trypanosoma brucei gambiense

Solutions: